Types of HPLC Columns Used in Analysis
Have you ever thought about the different kinds of HPLC columns?
This separation method is helpful for various industrial tasks, such as testing water, finding the active ingredients in medicines, and analyzing lipids and fats. So it makes sense that it is used so much in scientific research, making medicines and biotech products, and testing the environment.
Columns that can handle various flow rates and levels of separation are required for the separation and isolation of analytes. Are you interested to know more?
We’ve got you! This article will discuss the different kinds of HPLC columns and their use.
Capillary HPLC Columns
A capillary column is a narrow tube used in analytical chromatography utilized as the stationary phase in capillary liquid and gas chromatography. Capillary columns typically have inner diameters of 0.25 to 0.53 mm.
They are often covered with a fixed phase and formed of quartz or fused silica. The column length typically ranges from 30 to 150 meters.
Agilent HPLC Columns
Agilent HPLC Columns are columnar devices used in high-performance liquid chromatography (HPLC). They improve the efficiency and precision of HPLC separations of proteins.
Agilent HPLC Columns are made from various materials, including stainless steel, glass, and polymeric. The column’s dimensions and surface properties are essential to determine its performance.
Normal-Phase and Reverse-Phase HPLC Columns
Normal-phase HPLC columns are made with a non-polar stationary phase and a polar mobile phase. This means that the stationary phase is less likely to retain compounds, while the mobile phase is more likely to maintain compounds.
Reverse-phase HPLC columns are made with a polar stationary phase and a non-polar mobile phase. This means that the stationary phase is more likely to retain compounds, while the mobile phase is less likely to maintain compounds.
Partition HPLC Columns
Partition HPLC columns are a special kind of column used in HPLC and have a wall between the two phases. This divider makes it possible to separate the two phases.
It gives the sample a way to move through the column. Use them to separate many sample components, such as when analyzing food and drinks.
Ion-Exchange HPLC Columns
An Ion-Exchange column is used to take charged molecules out of a solution. This is done by running a solution with charged molecules through a column.
The molecules are drawn to the column ions with the opposite charge. A solution with the opposite charge then washes them away. This type of column helps separate proteins, carbohydrates, and other biomolecules.
Separate Those Sample Components!
Without high-performance liquid chromatography columns, it would be impossible to perform critical analyses in many scientific and industrial fields. The wide variety of HPLC column types and sizes available allows HPLC for different kinds of analyses.
Whether you are looking for a particular compound, trying to purify a sample, or want to know what is in a sample, HPLC can provide the answer. Your HPLC column supplier should be able to give you the information you need to make an informed decision.
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